Glycan microarrays for decoding the glycome
WebA large variety of glycans is intricately located on the cell surface, and the overall profile (the glycome, given the entire repertoire of glycoconjugate-associated sugars in cells and tissues) is believed to be crucial for the diverse roles of glycans, which are mediated by specific interactions that control cell-cell adhesion, immune response, microbial … WebMass Spectrometric and Glycan Microarray–Based Characterization of the Filarial Nematode Brugia malayi Glycome Reveals Anionic and Zwitterionic Glycan Antigens
Glycan microarrays for decoding the glycome
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WebJan 30, 2014 · A major technical breakthrough in glycosciences that facilitated decoding glycan functions was the development of printed glycan microarrays, in which many defined glycan structures are simultaneously presented to GBPs or microorganisms including viruses and bacteria. WebGlycan microarrays provide a high-throughputHigh-throughput technology for rapidly profiling interactions between carbohydrates and glycan-binding proteins (GBPs)Glycan-binding proteins (GBPs). ... Rillahan CD, Paulson JC (2011) Glycan microarrays for decoding the glycome. Annu Rev Biochem 80(1):797–823. https: ...
WebLectin microarrays, in which carbohydrate-binding proteins are probes for glycan structure, provide a systems-level view of the glycome (Fig. 1A) (18–20).These microarrays give specific information on the repertoire of glycans present, e.g., high mannose epitopes, branching patterns, and terminal α-2,3- or α-2,6-sialic acids, in a high-throughput format. WebGlycan microarrays for decoding the glycome. Annu Rev Biochem. 2011; 80:797–823. [PubMed: 21469953] 19. de Boer AR, Hokke CH, Deelder AM, Wuhrer M. General microarray technique for immobilization and screening of natural glycans. Analytical chemistry. 2007; 79:8107–8113. [PubMed: 17922555] Author Manuscript 20. ...
WebIn the last decade, glycan microarrays have revolutionized the analysis of the specificity of glycan-binding proteins (GBPs), providing information that simultaneously illuminates … WebFirst, the glycome can be deconstructed into an inventory of glycan structures separated from their protein or lipid scaffolds and independent of their location in the cell, organ, or organism. This first hierarchical level is essentially a catalog of structures. It is an important starting point for any comprehensive glycome analysis.
WebDec 5, 2010 · Abstract. Major challenges of glycomics are to characterize a glycome and identify functional glycans as ligands for glycan-binding proteins (GBPs). To address …
WebHere, we describe the methods to fabricate custom glycan microarrays that are used to examine glycan–GBP binding specificities. Conjugation-ready synthetic glycans are covalently attached to microarray surfaces through nucleophilic linker moieties. comfort suites north bergen njWebIn the last decade, glycan microarrays have revolutionized the analysis of the specificity of glycan-binding proteins (GBPs), providing information that simultaneously illuminates … dr william ware mystic ctWebGlycans and glycan binding proteins (GBPs or lectins) are essential components in almost every aspect of immunology. Investigations of the interactions between glycans and GBPs have greatly advanced our … comfort suites new york cityWebLectin microarray (LMA) is a high-sensitive glycan analysis technology used to obtain global glycomic profiles of both N- and O-glycans attached not only to purified glycoproteins but also to crude glycoprotein samples. Through additional use of laser microdissection (LMD) for tissue collection, we … comfort suites northlakeWebJul 12, 2013 · Search life-sciences literature (Over 39 million articles, preprints and more) comfort suites north ocalaWeb16 rows · Jan 1, 2011 · The advances in glycan array technology have paralleled those of complementary technologies used ... dr william watkins csunWebFeb 14, 2013 · Defined glycan microarrays are limited to the glycans we have available for printing on an array, and in many instances a defined array is missing important … dr william washington seattle