Dethiobiotin synthase

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August undefined, 2024

WebJan 23, 2007 · Dethiobiotin synthase 1 UniRule annotation. Gene names. Name. bioD1 UniRule annotation. Ordered locus names. b0778, JW0761. Organism names. Organism. Escherichia coli (strain K12) Taxonomic identifier. 83333 NCBI. Taxonomic lineage. Bacteria > Proteobacteria > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > … WebNov 1, 2012 · Biotin synthase is an S-adenosyl-l-methionine (SAM or AdoMet) radical enzyme that catalyzes reduction of the AdoMet sulfonium to produce 5′-deoxyadenosyl radicals, high-energy carbon radicals that can directly …

ATP-dependent dethiobiotin synthetase BioD 1 DrugBank Online

WebDethiobiotin synthetase Methods Enzymol. 1979;62:348-52. doi: 10.1016/0076-6879 (79)62241-3. Authors M A Eisenberg , K Krell PMID: 374977 DOI: 10.1016/0076-6879 … In enzymology, a dethiobiotin synthase (EC 6.3.3.3) is an enzyme that catalyzes the chemical reaction ATP + 7,8-diaminononanoate + CO2 ADP + phosphate + dethiobiotin The 3 substrates of this enzyme are ATP, 7,8-diaminononanoate, and CO2, whereas its 3 products are ADP, phosphate, and dethiobiotin. dustbusters reading ma

Biotin synthase from Escherichia coli: isolation of an enzyme …

WebMycobacterium tuberculosis dethiobiotin synthase (MtDTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. tuberculosis. … WebNov 18, 2005 · Working model for the compartmentation of biotin metabolism in the plant cell. Biotin-synthesizing enzymes are as follows: 1, KAPA synthase; 2, DAPA aminotransferase; 3, dethiobiotin synthase; and 4, biotin synthase. The occurrence of reaction 2 in the cytosol and of reaction 3 in mitochondria is predicted from DNA … Webdethiobiotin synthase. Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer. bioD dethiobiotin synthase [] Gene ID: 1245251, discontinued on 29-Dec-2024. Summary. Other designations. dethiobiotin synthase ... dustcatchers.com

Biotin, a universal and essential cofactor: synthesis, ligation and ...

RCSB PDB - 1BYI: STRUCTURE OF APO-DETHIOBIOTIN SYNTHASE …

WebOct 15, 1998 · The crystal structure of the 224-residue protein dethiobiotin synthetase from Escherichia coli has been refined using X-ray diffraction data at 0.97 A resolution at 100 K. The model, consisting of 4143 protein atoms including 1859 H atoms and 436 solvent sites, was refined to a final R factor of 11.6% for all reflections, and has an estimated ... WebBiotin synthase, a member of the ‘radical SAM’ (S-adenosylmethionine) family, converts DTB (dethiobiotin) into biotin. The active form of the Escherichia coli enzyme contains two (Fe-S ... dvd cheap buy moviesWebKrell K, Eisenberg MA: The purification and properties of dethiobiotin synthetase. J Biol Chem. 1970 Dec 25;245(24):6558-66. Yang G, Sandalova T, Lohman K, Lindqvist Y, Rendina AR: Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties. dvd cheap price

"WebJan 11, 2024 · BioD, dethiobiotin synthase In contrast to the preceding enzymes (with the exception of BioU), BioD (DTB synthase) catalyzes an unusually interesting step. This is the mechanistically unusual reaction of ATP (or CTP) dependent insertion of CO 2 between the N7 and N8 nitrogen atoms of DAPA to form the ureido moiety of biotin. " - Dethiobiotin synthase

Dethiobiotin synthase

RCSB PDB - 7JT5: Mycobacterium tuberculosis dethiobiotin …

WebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin … WebDethiobiotin. The conversion of dethiobiotin (4) into biotin (5), which is catalyzed by biotin synthase, is the most novel step in biotin biosynthesis since it involves the formal …

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WebMar 9, 2024 · biotin synthase. GeneRIFs: Gene References Into Functions. In biotin synthase, abstraction of a hydrogen atom from the C9 methyl group of dethiobiotin results in formation of a dethiobiotinyl methylene carbon radical, which is quenched by a sulfur atom to form a new carbon-sulfur bond in 9-mercaptodethiobiotin. WebBiotin synthase (BioB) catalyzes the oxidative insertion of a sulfur atom between the C6 methylene and the C9 methyl positions in dethiobiotin. The enzyme couples oxidation …

WebAug 17, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. … WebThis article is published in Journal of the American Chemical Society.The article was published on 1952-06-01. It has received 43 citation(s) till now.

Webdethiobiotin synthase. DTB synthetase. (7R,8S)-7,8-diammoniononanoate + ATP + CO2 <=> (4R,5S)-dethiobiotin + ADP + 3 H (+) + phosphate. CTP has half the activity of … WebAug 17, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. tuberculosis .Here, we report a binder of Mt DTBS, cyclopentylacetic acid 2 ( K D = 3.4 ± 0.4 mM), identified via in silico screening. X-ray crystallography showed that 2 binds in the …

WebJun 13, 2005 · Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. Gene Name bioB Uniprot …

WebMay 1, 2016 · The function of biotin synthase is to convert dethiobiotin, a product of dethiobiotin synthase (encoded by bioD), the third enzyme in the biotin biosynthesis pathway, to biotin. Biotin is an indispensable nutrient found in all living cells. Microorganisms, plants and a few fungi (most fungi obtain it from the environment) are … dvd checking softwareBiotin synthase (BioB) (EC 2.8.1.6) is an enzyme that catalyzes the conversion of dethiobiotin (DTB) to biotin; this is the final step in the biotin biosynthetic pathway. Biotin, also known as vitamin B7, is a cofactor used in carboxylation, decarboxylation, and transcarboxylation reactions in many organisms including humans. Biotin synthase is an S-Adenosylmethionine (SAM) dependent enzym… dustcarve dig flight rising dustbusters wildland firefightingWebJan 13, 1994 · Strands 1, 4, 5, and 6 form the classic mononucleotide-binding motif as found in other ATP- and GTP-binding proteins. Dethiobiotin synthase from E. coli is a dimer built up of two identical subunits. The dimer is formed through packing along a molecular two-fold axis, which in the crystals coincides with the crystallographic two-fold axis. dustbusters walmartWebMar 28, 1995 · One of these enzymes, ATP-dependent dethiobiotin synthetase, catalyzes the carboxylation of 7,8-diaminopelargonic acid leading to the formation of the ureido ring of biotin. The enzyme belongs to the class of ATP-dependent carboxylases and we present here the first crystal structure determined for this class of enzyme. dvd cheapestWebMay 1, 2005 · Biotin synthase is an S-adenosyl-L-methionine (SAM) radical enzyme that inserts sulfur into dethiobiotin to produce biotin. The reaction proceeds through 5′-deoxyadenosyl radical intermediates that become reduced during the sulfur insertion step to give another product of the reaction, 5′-deoxyadenosine. We report that Escherichia coli … dustcatchers incWebDethiobiotin synthase is involved in biotin biosynthesis and catalyses the reaction (CO2 + 7,8-diaminononanoate + ATP = dethiobiotin + phosphate + ADP). The enzyme binds ATP (see motif in first 12 residues of the SEED alignment) and requires magnesium as a co-factor. [Biosynthesis of cofactors, prosthetic groups, and carriers, Biotin] dvd cheaper by the dozen